Journal: Discover Oncology
Article Title: Lipid metabolism-related genes correlate with immune microenvironment and regulate the efficacy of immunotherapy via ferroptosis in melanoma
doi: 10.1007/s12672-025-04163-x
Figure Lengend Snippet: Overexpression ACSL4 increases the therapeutic efficacy of anti-PD-1 antibody in vivo. (A) A schematic view of the treatment plan, (B) Images of isolated tumors from xenograft mice that received indicated treatment. Tumor volumes and weights in each group were calculated and displayed on the right. (C) Immunofluorescence staining of ferroptosis marker PTGS2 in isolated xenograft tumors with indicated treatment. Scale bar = 100 μm. Data represent the mean ± SEM of triplicates. P value was calculated by two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001. n = 6
Article Snippet: The primary antibodies and dilutions for western blotting (WB) and immunofluorescence (IF) staining analysis are listed below: ACSL4 (22401-1-AP, Proteintech, Wuhan, China; 1:100 for IHC, 1:100 for IF and 1:5 000 for WB), ALOX5 (10021-1-Ig, Proteintech; 1:400 for IHC, and 1:500 for WB), PTGS2 (66351-1-Ig, Proteintech; 1:400 for IF), CD8α(ab22378,, Abcam; 1:300 for IF), ACTIN (66009-1-Ig, Proteintech; 1:10 000 for WB).
Techniques: Over Expression, Drug discovery, In Vivo, Isolation, Immunofluorescence, Staining, Marker, Two Tailed Test